Journal: Infection and Immunity

Article Title: STAT3 Promotes Schistosome-Induced Liver Injury by Inflammation, Oxidative Stress, Proliferation, and Apoptosis Signal Pathway

doi: 10.1128/iai.00309-20

Figure Lengend Snippet: Figure 3 JAK2/STAT3 signal pathway was activated in the liver of mice with S. 623

Article Snippet: After dissolution in 10% or 12% SDS-PAGE, 433 the total protein samples were transferred to nitrocellulose membranes for 2 h with 434 transfer buffer and blocked with 5% nonfat dried milk, which were dissolved in TBST 435 (20 mmol/L Tris–HCl, pH 7.6, 150 mmol/L NaCl and 0.02% Tween 20) for 1 h. 436 Membranes were incubated with primary antibodies for GAPDH (Cell Signaling 437 Technology-2118, 1:1500 dilution), α-SMA (abcam-ab5694,1:800), IL-6 438 (abcam-ab6672), p-JAK2 (Cell Signaling Technology-3776), JAK2 (Cell Signaling 439 Technology-3230), p-STAT3 (Cell Signaling Technology-9145), STAT3 (Cell 440 on D ecem ber 17, 2020 at 43709427 http://iai.asm .org/ D ow nloaded from 21 Signaling Technology-9139/4904), Bax (Santa Cruz Biotechnology-SC493), Bcl-2 441 (Santa Cruz Biotechnology-SC492), Sirt3 (Cell Signaling Technology-5490s), 442 Acetylated SOD2 (K68) (abcam-ab137037), SOD2 (abcam-ab13534) overnight at 443 4°C.

Techniques:

Journal: Infection and Immunity

Article Title: STAT3 Promotes Schistosome-Induced Liver Injury by Inflammation, Oxidative Stress, Proliferation, and Apoptosis Signal Pathway

doi: 10.1128/iai.00309-20

Figure Lengend Snippet: Figure 4 Liver p-STAT3 deficiency attenuates hepatic injury caused by S. 635

Article Snippet: After dissolution in 10% or 12% SDS-PAGE, 433 the total protein samples were transferred to nitrocellulose membranes for 2 h with 434 transfer buffer and blocked with 5% nonfat dried milk, which were dissolved in TBST 435 (20 mmol/L Tris–HCl, pH 7.6, 150 mmol/L NaCl and 0.02% Tween 20) for 1 h. 436 Membranes were incubated with primary antibodies for GAPDH (Cell Signaling 437 Technology-2118, 1:1500 dilution), α-SMA (abcam-ab5694,1:800), IL-6 438 (abcam-ab6672), p-JAK2 (Cell Signaling Technology-3776), JAK2 (Cell Signaling 439 Technology-3230), p-STAT3 (Cell Signaling Technology-9145), STAT3 (Cell 440 on D ecem ber 17, 2020 at 43709427 http://iai.asm .org/ D ow nloaded from 21 Signaling Technology-9139/4904), Bax (Santa Cruz Biotechnology-SC493), Bcl-2 441 (Santa Cruz Biotechnology-SC492), Sirt3 (Cell Signaling Technology-5490s), 442 Acetylated SOD2 (K68) (abcam-ab137037), SOD2 (abcam-ab13534) overnight at 443 4°C.

Techniques:

Journal: Infection and Immunity

Article Title: STAT3 Promotes Schistosome-Induced Liver Injury by Inflammation, Oxidative Stress, Proliferation, and Apoptosis Signal Pathway

doi: 10.1128/iai.00309-20

Figure Lengend Snippet: Figure 6 Schematic overview of JAK2/STAT3-mediated effects on S. japonicum 668

Article Snippet: After dissolution in 10% or 12% SDS-PAGE, 433 the total protein samples were transferred to nitrocellulose membranes for 2 h with 434 transfer buffer and blocked with 5% nonfat dried milk, which were dissolved in TBST 435 (20 mmol/L Tris–HCl, pH 7.6, 150 mmol/L NaCl and 0.02% Tween 20) for 1 h. 436 Membranes were incubated with primary antibodies for GAPDH (Cell Signaling 437 Technology-2118, 1:1500 dilution), α-SMA (abcam-ab5694,1:800), IL-6 438 (abcam-ab6672), p-JAK2 (Cell Signaling Technology-3776), JAK2 (Cell Signaling 439 Technology-3230), p-STAT3 (Cell Signaling Technology-9145), STAT3 (Cell 440 on D ecem ber 17, 2020 at 43709427 http://iai.asm .org/ D ow nloaded from 21 Signaling Technology-9139/4904), Bax (Santa Cruz Biotechnology-SC493), Bcl-2 441 (Santa Cruz Biotechnology-SC492), Sirt3 (Cell Signaling Technology-5490s), 442 Acetylated SOD2 (K68) (abcam-ab137037), SOD2 (abcam-ab13534) overnight at 443 4°C.

Techniques: